副教授

副教授
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王承健

姓名:王承健

所属部门:食品科学与工程学院

职务/职称:副教授

荣誉硕士生导师

电子邮件wangchj@nwu.edu.cn

 

个人简介

王承健,中共党员,博士,副教授,硕士生导师。主要从事食品生物化学与生物技术、食品营养健康与质量安全、食品相关复杂分子人工合成以及新型生物活性成分与特殊食品开发等领域的基础和应用基础研究,尤其在糖复合物生物活性寡糖链的定性定量分析、释放制备及功能机制研究方面建立了系列新技术。Carbohydrate Research杂志审稿人,中国生物化学与分子生物学会糖复合物专业委员会首届青年委员会委员。主持国家自然科学基金面上项目与青年基金、陕西省自然科学基础研究计划面上项目、西北大学“青年学术英才支持计划”项目等项目6项,在Analytical ChemistryJournal of Agricultural and Food ChemistryJournal of Proteome ResearchJournal of Chromatography ASCI期刊上发表论文30余篇,授权国家发明专利6项,获陕西省高等学校科学技术一等奖(排名第二)和陕西省优秀博士论文奖,被评为西北大学2022-2023教学年度优秀教师。

 

研究成果:

1. 科研项目

1)国家自然科学基金面上项目,基于吡唑啉酮功能化材料固相萃取技术的单糖糖基化蛋白的糖蛋白组学分析通用新方法研究,主持

2)国家自然科学基金青年基金,基于液质联用技术的糖蛋白O-糖链结构快速完全解析新方法研究,主持

3)陕西省自然科学基础研究计划一般项目(面上),新型固定化O-糖链分析方法及肠道菌群黏附机制研究,主持

4陕西省教育厅自然科学专项,糖蛋白寡糖链的非还原性释放和异二官能团荧光试剂同步衍生化新方法及应用研究,主持

5)西北大学“青年学术英才支持计划”,入选人

6)西北大学科研启动基金,糖蛋白寡糖链的氨基吡唑啉酮新型衍生物单体的制备方法研究,主持

 

2. 代表性论文

(1) Meiyi Zou, Yuxia Liu, Lijuan Man, Yao Lan, Qian Wei ... Chengjian Wang*. Comprehensive comparison of bioactive N-glycans among seven species of livestock and poultry plasma using a relative quantification strategy. Journal of Agricultural and Food Chemistry 2023. Accepted.

(2) 满丽娟, 叶佳佳, 张德锋......王承健*. 丝胶蛋白源TnT抗原的释放制备及质谱分析. 高等学校化学学报 2023. 已录用.

(3) Chengjian Wang, Xi Gao, Guiping Gong et al. A versatile strategy for high-resolution separation of reducing glycan mixtures as hydrazones by two-dimensional high-performance liquid chromatography. Journal of Chromatography A 2022, 1685, 463599.

(4) Wanjun Jin#, Chengjian Wang#, Meifang Yang, et al. Glycoqueuing: isomer-specific quantification for sialylation-focused glycomics. Analytical Chemistry 2019, 91(16), 10492–10500.

(5) Chengjian Wang, Yanan Wen, Meifang Yang, et al. High-sensitivity quantification of glycosphingolipid glycans by ESI-MS utilizing ozonolysis-based release and isotopic Girard’s reagent labeling. Analytical Biochemistry 2019, 582, 113355.

(6) Chengjian Wang, Yu Lu, Jianli Han, et al. Simultaneous release and labeling of O- and N-glycans allowing for rapid glycomic analysis by online LC-UV-ESI-MS/MS. Journal of Proteome Research 2018, 17, 2345-2357.

(7) Chengjian Wang, Shan Qiang, Wanjun Jin, et al. Reductive chemical release of N-glycans as 1-amino-alditols and subsequent 9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis. Journal of Proteomics 2018, 187, 47-58.

(8) Chengjian Wang, Meifang Yang, Xi Gao, et al. The ammonia-catalyzed release of glycoprotein N-glycans. Glycoconjugate Journal 2018, 35(4), 411-420.

(9) Yu Lu#, Chengjian Wang#, Rendan Liu, et al. 3-Amino-1-phenyl-2-pyrazoline-5-ketone as a heterobifunctional chromogenic reagent to derivatize reducing glycans for subsequent online LC/MS analysis. Analytical Biochemistry 2018, 549, 1-11.

(10) Chengjian Wang, Ping Zhang, Wanjun Jin, et al. Quantitative O-glycomics based on improvement of the one-pot method for nonreductive O-glycan release and simultaneous stable isotope labeling with 1-(d0/d5)phenyl-3-methyl-5-pyrazolone followed by mass spectrometric analysis. Journal of Proteomics 2017, 150, 18-30.

(11) Lingmei Li#, Chengjian Wang#, Shan Qiang, et al. Mass spectrometric analysis of Nglycoforms of soybean allergenic glycoproteins separated by SDS-PAGE. Journal of Agricultural and Food Chemistry 2016, 64, 7367−7376.

(12) Chengjian Wang, Zhiyu Wu, Jiangbei Yuan, et al. Simplified quantitative glycomics using the stable isotope label Girard’s reagent P by electrospray ionization mass spectrometry. Journal of Proteome Research 2014, 13, 372−384.

(13) Chengjian Wang, Jiangbei Yuan, Zhongfu Wang* & Linjuan Huang*. Separation of one-pot procedure released O-glycans as 1-phenyl-3-methyl-5-pyrazolone derivatives by hydrophilic interaction and reversed-phase liquid chromatography followed by identification using electrospray mass spectrometry and tandem mass spectrometry. Journal of Chromatography A 2013, 1274, 107–117.

(14) Chengjian Wang, Jiangbei Yuan, Xiaohua Li, et al. Sulfonyl hydrazine-functionalized polymer as a specific capturer of reducing glycans from complex samples for high-throughput analysis by electrospray ionization mass spectrometry. Analyst 2013, 138, 5344–5356.

(15) Chengjian Wang, Wancui Fan, Ping Zhang, et al. One-pot nonreductive O-glycan release and labeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis. Proteomics 2011, 11, 4229–4242.

 

3. 发明专利

1)一种通用低成本季铵盐类糖链同位素标记试剂及合成方法,ZL202011553376.7,已授权,第一发明人

2)还原性糖的不同吡唑啉酮试剂衍生物之间相互转化的方法,ZL201910164988.8,已授权,第一发明人

3)从还原性糖的肼类发色试剂衍生物中再生还原性糖的方法,ZL201810064494.8,已授权,第二发明人

4)唾液酸化糖链同分异构体的高分辨顺序分离和准确定量分析方法,ZL201811434664.3,已授权,第二发明人

5)还原性糖和糖蛋白O-糖链的氨基吡唑啉酮类异二官能团试剂衍生化及分离分析方法,ZL201710954115.8,已授权,第二发明人

6)还原性释放糖蛋白N-糖链及其衍生物分离和分析鉴定的方法,ZL201710409524.X,已授权,第二发明人

 

获奖情况:

1)2017年陕西省优秀博士论文

2)2017年陕西高等学校科学技术奖一等奖(第2完成人)

 

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