所属部门：食品科学与工程学院

　　职务/职称：教授

　　荣誉：教育部“新世纪优秀人才支持计划”人选，博士生导师

　　电子邮件：huanglj@nwu.edu.cn

　　个人简介

　　黄琳娟，博士，教授，博士生导师。1993年本科毕业陕西师范大学化学系，1999年博士毕业于中科院上海有机化学研究所，2000-2004年在美国密歇根州立大学做博士后研究，2004年回国到西北大学生命科学学院工作，2018年至今在西北大学食品科学与工程学院任教。研究领域主要集中在碳水化合物化学与营养学方向领域，包括功能寡糖、糖缀合物的分离，结构解析及其功能活性研究;复杂碳水化合物分析鉴定新技术、新方法研究。近年来，在Journal of Agricultural and Food Chemistry, Food chemistry, Journal of Ethnopharmacology, Journal of Proteome Research, Analyst, Proteomics, Glycobiology, Analytical Biochemistry, Journal of Chromatography A, Carbohydrate Research 等国内外重要期刊发表研究论文70余篇。已培养博士毕业生6人，其中两人的博士毕业论文被评为陕西省优秀博士论文。主持国家自然科学基金面上项目4项，国家“863”项目1项，省部级项目多项。

　　联系方式：029-88302655

　　通讯地址：陕西省西安市碑林区太白北路229号食品科学与工程学院

　　邮编：710069

　　电子邮件：huanglj@nwu.edu.cn

　　研究成果

　　1. Xiaoyi Chen, Rui Tang, Tingting Liu, Wei Dai, Qian Liu, Guiping Gong, Shuang Song, Minghua Hu, Linjuan Huang, Zhongfu Wang. Physicochemical properties, antioxidant activity and immunological effects in vitro of polysaccharides from Schisandra sphenanthera and Schisandra chinensis. International Journal of Biological Macromolecules (2019), 131: 744–751.

　　2. Yu Lu, Cheng Li, Ming Wei, Yue Jia, Jingjing Song, Ying Zhang, Chengjian Wang, Linjuan Huang, Zhongfu Wang. Release, Separation, and Recovery of Monomeric Reducing N‑Glycans with Pronase E Combined with 9‑Chloromethyl Chloroformate and Glycosylasparaginase. Biochemistry (2019), 58: 1120−1130.

　　3. Gong, Guiping; Dang, Tiantian; Deng, Yangni; Han, Jianli; Zou, Zihua; Jing, Sun; Zhang, Ying; Liu, Qian; Huang, Linjuan; Wang, Zhongfu. Physicochemical properties and biological activities of polysaccharides from Lycium barbarum prepared by fractional precipitation. International Journal of Biological Macromolecules (2018), 109, 611-618.

　　4. Wang, Chengjian; Zhang, Ping; Jin, Wanjun; Li, Lingmei; Qiang, Shan; Zhang, Ying; Huang, Linjuan; Wang, Zhongfu. Quantitative O-glycomics based on improvement of the one-pot method for nonreductive O-glycan release and simultaneous stable isotope labeling with 1-(d0/d5)phenyl-3-methyl-5-pyrazolone followed by mass spectrometric analysis. Journal of Proteomics (2017), 150, 18-30.

　　5. Li, Lingmei; Wang, Chengjian; Qiang, Shan; Zhao, Jixiang; Song, Shuang; Jin, Wanjun; Wang, Bo; Zhang, Ying; Huang, Linjuan; Wang, Zhongfu. Mass Spectrometric Analysis of N-Glycoforms of Soybean Allergenic Glycoproteins Separated by SDS-PAGE. Journal of Agricultural and Food Chemistry (2016), 64(39), 7367-7376.

　　6. Sun, Yujiao; Sun, Wei; Guo, Jiatong; Hu, Xiuting; Gong, Guiping; Huang, Linjuan; Cao, Hongzhi; Wang, Zhongfu. Sulphation pattern analysis of chemically sulphated polysaccharide LbGp1 from Lycium barbarum by GC-MS. Food Chemistry (2015), 170, 22-29.

　　7. Zhang, Ying; Wang, Chengjian; Liu, Yang; Yao, Weinan; Sun, Yujiao; Zhang, Ping; Huang, Linjuan; Wang, Zhongfu. Fluorescein-5-thiosemicarbazide (FTSC) labeling for fluorescent imaging of pectin-derived oligogalacturonic acid transported in living cells by confocal microscopy. European Food Research and Technology (2014), 239(5), 867-875.

　　8. Jiang, Kuan; Wang, Chengjian; Sun, Yujiao; Liu, Yang; Zhang, Ying; Huang, Linjuan; Wang, Zhongfu. Comparison of Chicken and Pheasant Ovotransferrin N-Glycoforms via Electrospray Ionization Mass Spectrometry and Liquid Chromatography Coupled with Mass Spectrometry. Journal of Agricultural and Food Chemistry (2014), 62(29), 7245-7254.

　　9. Wang, Zhongfu; Liu, Yang; Sun, Yujiao; Mou, Qing; Wang, Bo; Zhang, Ying; Huang, Linjuan. Structural characterization of LbGp1 from the fruits of Lycium barbarum L. Food Chemistry (2014), 159, 137-142.

　　10. Sun, Yujiao; Liu, Yang; Jiang, Kuan; Wang, Chengjian; Wang, Zhongfu; Huang, Linjuan. Electrospray Ionization Mass Spectrometric Analysis of κ-Carrageenan Oligosaccharides Obtained by Degradation with κ-Carrageenase from Pedobacter hainanensis. Journal of Agricultural and Food Chemistry (2014), 62(11), 2398-2405.

　　11. Wang, Chengjian; Wu, Zhiyu; Yuan, Jiangbei; Wang, Bo; Zhang, Ping; Zhang, Ying; Wang, Zhongfu; Huang, Linjuan. Simplified Quantitative Glycomics Using the Stable Isotope Label Girard's Reagent P by Electrospray Ionization Mass Spectrometry. Journal of Proteome Research (2014), 13(2), 372-384.

　　12. Wang, Chengjian; Yuan, Jiangbei; Li, Xiaohua; Wang, Zhongfu; Huang, Linjuan. Sulfonyl hydrazine-functionalized polymer as a specific capturer of reducing glycans from complex samples for high-throughput analysis by electrospray ionization mass spectrometry. Analyst (2013), 138(18), 5344-5356.

　　13. Zhang, Ying; Yuan, Jiangbei; Song, Jingjing; Wang, Zhongfu; Huang, Linjuan. An efficient method for selectively imaging and quantifying in situ the expression of sialylated glycoproteins on living cells. Glycobiology (2013), 23(6), 643-653.

　　14. Wang, Chengjian; Yuan, Jiangbei; Wang, Zhongfu; Huang, Linjuan. Separation of one-pot procedure released O-glycans as 1-phenyl-3-methyl-5-pyrazolone derivatives by hydrophilic interaction and reversed-phase liquid chromatography followed by identification using electrospray mass spectrometry and tandem mass spectrometry. Journal of Chromatography A (2013), 1274, 107-117.

　　15. Lv, Xiaopeng; Wang, Chengjian; Cheng, Yang; Huang, Linjuan; Wang, Zhongfu. Isolation and structural characterization of a polysaccharide LRP4-A from Lycium ruthenicum Murr. Carbohydrate Research (2013), 365, 20-25.

　　16. Peng, Qiang; Song, Jingjing; Lv, Xiaopeng; Wang, Zhongfu; Huang, Linjuan; Du, Yuguang. Structural Characterization of an Arabinogalactan-Protein from the Fruits of Lycium ruthenicum. Journal of Agricultural and Food Chemistry (2012), 60(37), 9424-9429.

　　17. Zhang, Ying; Sun, Yujiao; Wang, Zhongfu; Huang, Linjuan. Fluorescein-5-thiosemicarbazide as a probe for directly imaging of mucin-type O-linked glycoprotein within living cells. Glycoconjugate Journal (2012), 29(5-6), 445-452.

　　18. Wang, Chengjian; Fan, Wancui; Zhang, Ping; Wang, Zhongfu; Huang, Linjuan. One-pot nonreductive O-glycan release and labeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis. Proteomics (2011), 11(21), 4229-4242.

　　19. Zhang, Ying; Wang, Zhongfu; Zhang, Xiaorui; Zhou, Wenxia; Huang, Linjuan. One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells. Carbohydrate Research (2011), 346(14), 2156-2164.

　　20. Zhang, Ping; Zhang, Ying; Xue, Xiangdong; Wang, Chenjian; Wang, Zhongfu; Huang, Linjuan. Relative quantitation of glycans using stable isotopic labels 1-(d0/d5) phenyl-3-methyl-5-pyrazolone by mass spectrometry. Analytical Biochemistry (2011), 418(1), 1-9.

　　获奖情况

　　1. 《寡糖/多糖分析鉴定方法和技术体系的建立及应用》，陕西高等学校科学技术奖，一等奖，黄琳娟(3/11)。(2017)

　　2. 入选教育部“新世纪优秀人才支持计划”(2008)